qPCR timecourse
The following layout was used in a qPCR experiment to measure the change in GFP expression (compared to the 16S reference gene) over time in different ligand conditions. Note that the TOML file has very little redundancy, even though the layout isn’t particularly regular.
[expt]
sgrna = 'ligRNA-'
[block.4x3.A1]
time = 00:00:00
[block.8x3.A9]
time = 00:02:00
[block.8x3.D1]
time = 00:04:20
[block.8x3.D9]
time = 00:07:00
[block.8x3.G1]
time = 00:10:00
[block.8x3.G9]
time = 00:13:20
[block.8x3.J1]
time = 00:17:00
[block.8x3.J9]
time = 00:21:00
[block.8x3.M1]
time = 00:25:20
[block.8x3.M9]
time = 00:30:00
[col.'1,3,...,17']
primers = 'gfp'
[col.'2,4,...,18']
primers = '16s'
[block.2x3.A1]
ligand = 'apo'
[block.2x3.A3]
ligand = 'holo'
[block.2x12.D1]
ligand = 'apo→apo'
[block.2x15.A9]
ligand = 'apo→apo'
[block.2x12.D3]
ligand = 'apo→holo'
[block.2x15.A11]
ligand = 'apo→holo'
[block.2x12.D5]
ligand = 'holo→apo'
[block.2x15.A13]
ligand = 'holo→apo'
[block.2x12.D7]
ligand = 'holo→holo'
[block.2x15.A15]
ligand = 'holo→holo'
# Controls:
[block.2x3.A5]
control = 'no GFP'
[block.2x3.A7]
control = 'no RT'
[block.2x3.A17]
control = 'no cDNA'
